Sequence-specific Polymerase Chain-reaction Markers Derived from Randomly Amplified Polymorphic DNA Markers for Fingerprinting Grape (Vitis) Rootstocks
نویسندگان
چکیده
منابع مشابه
Study of Genetic Diversity among Philodendron Varieties by Randomly Amplified Polymorphic DNA Markers
In the present study, Randomly Amplified Polymorphic DNA (RAPD) markers were used to analyze the genetic diversity in 20 varieties of Philodendron. The polymerase chain reaction was performed with 60 RAPD primers, out of which 21 primers showed clear amplification as well as more polymorphism. In total, 354 scorable RAPD loci with 348 polymorphic bands (98%) were observed. Percentages of polymo...
متن کاملSerogrouping and Randomly Amplified Polymorphic DNA Fingerprinting of Campylobacter Jejuni
Results: Twenty one C. jejuni strains belonged to the following Penner’s serogroups: 8 strains were group A, 13 strains were group O. The remaining 5 strains were non-typable. RAPD-PCR analysis of 26 strains of Campylobacter jejuni yielded multiple amplification products in all of them. None of the strains processed by this method was non-typable. According to the number and sizes of amplificat...
متن کاملEvaluation of Genetic Variability in a Breeder Flock of Native Chicken Based on Randomly Amplified Polymorphic DNA Markers
A study was undertaken to evaluate the genetic variation in the 10th generation of a breeder flock of native breed selected for high egg and meat production in native fowls breeding station, Mazandaran, Iran. Venous blood samples were collected from 100 birds of both sexes. The RAPD-PCR technique was applied to generate a DNA fingerprint of individuals. Initially, a total of 20 ten-nucleotide a...
متن کاملRandom Amplified Polymorphic DNA (RAPD) Markers
Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplificatio...
متن کاملstudy of genetic diversity among philodendron varieties by randomly amplified polymorphic dna markers
in the present study, randomly amplified polymorphic dna (rapd) markers were used to analyze the genetic diversity in 20 varieties of philodendron. the polymerase chain reaction was performed with 60 rapd primers, out of which 21 primers showed clear amplification as well as more polymorphism. in total, 354 scorable rapd loci with 348 polymorphic bands (98%) were observed. percentages of polymo...
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ژورنال
عنوان ژورنال: Journal of the American Society for Horticultural Science
سال: 1995
ISSN: 0003-1062,2327-9788
DOI: 10.21273/jashs.120.5.714